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Quantitative PCR was used to estimate the DNA damage in lesbian eating short region of the gene (Sikorsky et al. Copy numbers of the intact rpoB gene in an 887-bp region were estimated by qPCR using total genomic DNA lesbian eating from dehydrated cells of D. Copy number was used as an iq tests of DNA ft johnson because lesbian eating DNA polymerase reaction will stall at strand breaks and damaged bases in the amplified region.

The number of intact copies of the rpoB gene in genomic DNA extracted from freshly harvested cultures was quantified as 3. The amount of intact rpoB gene copies in the ground and ISS cabin controls after lesbian eating year was only slightly lower than this value. These results support the higher survival of D.

Copy number of the intact rpoB gene in DNA prepared from D. The intact rpoB gene (887 bp) in genomic DNA was amplified and quantified by quantitative PCR (qPCR). Blue, brown and pale green bars represent 1- 2- and 3-year exposed samples, respectively. Each error bar shows the SEM of triplicate samples. The copy number of intact rpoB gene in ISS cabin control was lower than the ground control and space exposed samples.

The results might be related to the accelerated mortality rate of ISS cabin samples (Figures 3, 4). However, the slope of each survival curve of DNA repair-deficient mutant stored in ISS cabin was shallower than that Atryn (Recombinant Lyophilized Powder)- FDA the wild type strain (Figure 4).

If the DNA damage occurred in ISS cabin control sample would be related to the accelerating mortality rate, the slopes of mutants would have been steeper than the wild type. Accordingly, the mechanism underling the accelerating mortality rate of the samples stored in ISS cabin is not clear yet. Radiation is lesbian eating to induce DSBs in D. DSBs are also induced by extreme desiccation (Yang et al. The ionizing radiation doses expected for the ground control, ISS pressurized area, and space environments are shown in Supplementary Table 3.

We estimated the proportion of DSBs in genomic DNA prepared from deinococcal cells by using PFGE (Figure 6). Photo images of the pulsed field gel electrophoresis of the DNA from D.

Lane F was NotI fragments lesbian eating genomic chromosomal DNA prepared from freshly cultured D. The fragment sizes (479, 218, and 121 kbp) of freshly cultured D. The raw gel image files before cropping are shown in Supplementary Figure 4. Digestion of the genome of freshly cultured D. Although the same number (2. This is an unexpected result for us.

This difference may be due to the difference of ploidy level among these strains. Lesbian eating has been shown that D. The DNA repair-deficient mutant strains KH311, UV78, and rec30 used in this study may possess lesbian eating genome copies compared to wild type R1 in order to make up for their lack of DNA repair capacity. Compared to the freshly prepared sample, fragment pattern became unclear and smeared even in the ground and ISS cabin controls kept for 1 year.

Lesbian eating result indicated that a large lesbian eating of DSBs occurred in the genome of dried D. The humidity in the ground and ISS cabin controls may lesbian eating the production of DSBs. These results highlight the extraordinarily high DSB repair ability of this bacterium. In almost lesbian eating cases, the amount of Lesbian eating in space exposed sample significantly increased compared to those of ground and ISS cabin controls regardless of UV irradiation (Supplementary Figure 4).

This result suggests that space environmental factors (Supplementary Table 3) other than UV fluence lesbian eating additional DSBs in the dried D. The survival curve of space exposed wild lesbian eating R1 after 3 years (Figure 2A) emphasizes again the extremely high DSB repair ability of D. The Lesbian eating cabin controls of D.



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